Ability of a salivary intrinsically unstructured protein to bind different tannin targets revealed by mass spectrometry |
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Authors: | Francis Canon Alexandre Giuliani Franck Paté Pascale Sarni-Manchado |
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Institution: | (1) Laboratory of Bioanalytical Chemistry, Department of Analytical Chemistry, University of Torino, via Giuria 5, 10125 Turin, Italy |
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Abstract: | Superporous monolithic hydrogels (cryogel monoliths) are elastic, sponge-like materials that can be prepared in an aqueous
medium through a cryotropic gelation technique. These monoliths show interesting properties for the development of high-throughput
solid-phase extraction supports to treat large volumes of aqueous samples. In this work, a cryogel-supported molecularly imprinted
solid-phase extraction approach for the endocrine disruptor bisphenol A (BPA) from river water and wine samples is presented.
An imprinted polymer with molecular recognition properties for BPA was prepared in acetonitrile by thermal polymerization
of a mixture of 4,4′-dihydroxy-2,2-diphenyl-1,1,1,3,3,3-trifluoropropane as a mimic template of BPA, 4-vinylpyridine and trimethylolpropane
trimethacrylate in a molar ratio of 1 + 6 + 6. Fine imprinted particles (<10 μm) were embedded in a poly-acrylamide-co-N,N′-methylenbisacrylamide cryogel obtained by ammonium persulfate-induced cryopolymerization at −18 °C. The resulting monolithic
gel was evaluated for its use as a sorbent support in an off-line solid-phase extraction approach to recover BPA from dilute
aqueous samples with minimum pre-loading work-up. The optimized extraction protocol resulted in a reliable MISPE method suitable
to selectively extract and preconcentrate BPA from river water and red wine samples, demonstrating the practical feasibility
of cryogel-trapped imprinted polymers as solid-phase extraction materials |
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