| 扫描电子显微镜叔丁醇冷冻干燥快速制样方法的探索 |
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| 引用本文： | 刘乐,王文可,卓静,唐娜,杨晴晴,李佳怡,刘飞红,王梦鸽,袁金红.扫描电子显微镜叔丁醇冷冻干燥快速制样方法的探索[J].电子显微学报,2022,41(1):98-104. |
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| 作者姓名： | 刘乐 王文可 卓静 唐娜 杨晴晴 李佳怡 刘飞红 王梦鸽 袁金红 |
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| 作者单位： | 河南师范大学生命科学学院,河南 新乡453007;河南师范大学生命科学学院,河南 新乡453007;河南省高校道地中药材保育及利用工程技术研究中心,河南 新乡453007 |
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| 基金项目： | 国家自然科学基金资助项目(Nos.31970380,31670317)； |
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| 摘 要： | 该实验旨在利用扫描电子显微镜(SEM)进行植物叶片微观形态观察,探究一种叔丁醇快速制样新方法.实验以野生型拟南芥叶片为研究对象,通过预设实验步骤对其进行制样处理,结合实验过程及观察结果总结最佳实验处理条件作为新的SEM叔丁醇快速制样方法.实验结果显示,在不同的叔丁醇处理温度、时长,冰冻时间及喷金导电处理条件下,叶片表皮...
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| 关 键 词： | SEM制样 拟南芥叶片 微观形态观察 |
Studies on scanning electron microscope method for rapid leaf sample preparation with tert-butanol |
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| LIU Le,WANG Wen-ke,ZHUO Jing,TANG Na,YANG Qing-qing,LI Jia-yi,LIU Fei-hong,WANG Meng-ge,YUAN Jin-hong.Studies on scanning electron microscope method for rapid leaf sample preparation with tert-butanol[J].Journal of Chinese Electron Microscopy Society,2022,41(1):98-104. |
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| Authors: | LIU Le WANG Wen-ke ZHUO Jing TANG Na YANG Qing-qing LI Jia-yi LIU Fei-hong WANG Meng-ge YUAN Jin-hong |
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| Institution: | (College of Life Sciences,Henan Normal University,Xinxiang Henan 453007;Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs of Colleges and Universities in Henan Province,Xinxiang Henan 453007,China) |
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| Abstract: | The aim of this study was to explore the microscopic morphology of plant leaves by scanning electron microscopy(SEM), and to find a new method for rapid preparation of SEM leaf samples using tert-butanol. In this study, wild-type Arabidopsis leaves were prepared by preset experimental steps as the research object. Regarding the experimental process and based on the observation results, optimal experimental treatment conditions were summarized as a new SEM rapid sample preparation method for tert-butanol. The results indicated that there were differences in the microscopic morphology of leaf epidermis under different conditions of tert-butanol treatment temperature, duration, freezing time and gold-spraying conductive treatment, among which tert-butanol treatment for 15 min at 30 ℃, freezing on ice for 10 min, and gold-spraying 80 s had the best effect. In this study, a new method for rapid sample preparation of Arabidopsis thaliana leaves by SEM tert-butanol was established, and the experimental results showed that this method also had good observation effect on other species such as Dioscorea oppsita. |
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| Keywords: | SEM sample preparation Arabidopsis leaves micromorphological observation |
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