聚乙二醇功能化程度对纳米金壳夹心二氧化硅载药量及稳定性影响研究

本文系统研究了不同聚乙二醇(PEG)功能化程度对纳米金壳夹心二氧化硅(GSNs)载药量及稳定性的影响.透射电子显微镜(TEM)观察结果表明,GSNs表面经修饰分子量为5 KDa的α-甲氧基-ω-巯基聚乙二醇(mPEG-SH)分子后,PEG分子层平均厚度约为4 nm;随PEG化程度增加,GSNs聚集沉降速度改善,稳定性提高,久置后(35 d)稳定性良好;电感耦合等离子体发射光谱(ICP-OES)测试表明,当PEG与Au反应摩尔比为0.24时,PEG分子层在金壳表面接近饱和;不同PEG化程度对GSNs载药量基本无影响.本研究可为优化无机纳米材料表面PEG修饰过程,发展无机纳米载体材料体内应用提供理论依据.

The Influence of PEG Grafting Level on the Drug Loading Capacity and Stability of Gold Nanoshells on Silica Nanorattles

In this paper,we grafted various amounts of polyethylene glycol(PEG) onto the surface of gold nanoshells on silica nanorattles(GSNs) and investigated the effects of grafting level about drug loading capacity and stability of GSNs.The results showed that the average thickness of GSNs increased about 4 nm after PEGylation.With the increase of PEGylation,the sedimentation rate of GSNs decreased gradually and the stability increased,and GSNs keeps good stability after standing 35 days.Inductively coupled plasma atomic emission spectrometry(ICP-OES) tests show that it will reach saturation point of PEGylation when the molar ratio of PEG and Au reaches nearly 0.24.In addition,it has no influence on drug loading capacity about different PEGylation processes.The results of this study will provide an important theoretical basis for the optimization the PEGlyation on the surface of inorganic nanomaterials and for the development of inorganic nano-carrier materials in vivo applications.