纳米硫化钴与明胶蛋白质的原位键合作用研究

利用紫外-可见吸收光谱(UV-Vis)和傅里叶变换红外光谱(FT-IR),研究了pH值11.00时,不同温度下CoS纳米粒子与明胶蛋白质的键合作用.根据吸光度与CoS浓度的关系,由Lineweave-Burk方程计算了不同温度下CoS纳米粒子与明胶蛋白作用的键合常数K(温度为293 K时键合常数K为3.01×10³L/mol;温度为301 K时键合常数K为2.12×10³L/mol;温度为313 K时键合常数K为1.85×10³L/mol)以及对应温度下反应的热力学参数(ΔrH_m=-17.93 kJ/mol;ΔrS_m=4.93 J/(K.mol);ΔrG_m=-19.37/-19.41/-19.47kJ/mol).CoS纳米粒子与明胶蛋白之间主要靠静电力结合.研究结果为初步探索纳米颗粒与纤维状蛋白质之间相互作用的化学机制提供了必要的信息.

Study on the In Situ Binding of Nano-CoS to Gelatin

The in situ binding of CoS nanoparticles to gelatin was studied using UV-visible and FT-IR methods at pH 11.00 and different temperatures.The absorbance data were analyzed according to Lineweave-Burk double-reciprocal equation.K,the binding constants of the compound at different temperatures(293 K: 3.01×103L/mol;301 K: 2.12×103 L/mol;313 K: 1.85×103 L/mol) and the thermodynamic parameters(ΔrHm =-17.93 kJ/mol;ΔrSm =4.93 J/K·mol;ΔrGm =-19.37/-19.41/-19.47 kJ/mol) at correspondence temperatures were obtained.The negative enthalpy(ΔrHm) and positive entropy(ΔrSm) values indicate that electrostatic force played the major roles in the binding reaction of nano-CoS with gelatin.The results provide useful information for exploring the chemical mechanism of interaction between nanoparticle and fibrous protein.