Mycobacterium E3休止细胞催化烯烃立体选择性环氧化

初步研究了ＭｙｃｏｂａｃｔｅｒｉｕｍＥ３的生长和产酶特性，利用ＭｙｃｏｂａｃｔｅｒｉｕｍＥ３休止细胞催化烷羟化和烯烃环氧化，研究结果表明，烷烃不能被羟化，烯烃环氧化具有底物选择性，对烯丙基型底物ＸＣＨ２ＣＨ＝ＣＨ２（Ｘ＝Ｈ，Ｃｌ，Ｂｒ，ＯＨ）取代基大小显著性环氧化活性，ＭｙｃｏｂａｃｔｅｒｉｕｍＥ３中烯烃单加氧酶和另一种结构未知的酶的存在导致烯烃环氧化过程中存在环氧化物的立体选择性形成和非立体选择

Stereoselective Epoxidation of Alkenes Catalyzed by Mycobacterium E3 Resting Cell

Growth Characteristics of Mycobacterium E3 on ethene in mineral salts mediumand oxidation of alkanes and dsenes catalyzed by Mycobacterium E3 resting cell werestudied. Mycobacteriurn E3 was unable to, catalyze hydroxylation of alkanes, but ableto catalyze epoxidation of alkenes specifically. Influence of substituents on epoxidizingactivity was discussed for allyl substrates XCH2CH=CH2 (X=H, Cl, Br,OH). Alkenemonooxygenase and an unknown enzyme in Mycobacterium E3 catalyzed stereoselectiveepoxidation of alkenes and not-stereoselective degradation of epoaldes. Optical rotationand chiral capillary chromatography revealed that propene and 3-chloro-1-propene wereepoaldized to (R)-1,2-epoxypropane and (S)-1-chloro-2,3-epoxypropane.