A metallomics approach discovers selenium-containing proteins in selenium-enriched soybean |
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Authors: | Qilin?Chan Email author" target="_blank">Joseph?A?CarusoEmail author |
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Institution: | (1) Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221-0172, USA; |
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Abstract: | Our previous study found that high-molecular-weight selenium (Se) species make up 82% of the total Se in the bean of Se-enriched
soybean plants (Chan et al. 2010, Metallomics, 2(2): p. 147–153). The Se species have been commonly seen in other plants in addition to soybean, but their identities remain
unresolved. The present study employs a multi-technique metallomics approach to characterize the proteins containing Se in
the beans of Se-enriched soybean plants. Two main categories of proteins, maturation proteins and protease inhibitors, were
found in Se-containing high-performance liquid chromatography (HPLC) fractions. The proteins were screened by two-dimensional
HPLC-inductively coupled plasma mass spectrometry, size-exclusion chromatography, and anion-exchange chromatography, and the
Se-containing fractions were then identified by peptide mapping using HPLC-Chip-electrospray ion trap mass spectrometry. Based
on the belief that Se goes into proteins through non-specific incorporation, a new method was designed and applied for the
Se-containing peptide identification. The Se-containing peptide KSDQSSSYDDDEYSKPCCDLCMCTRS, part of the sequence of protein
Bowman–Birk proteinase isoinhibitor (Glycine max), was found in one of the Se-containing fractions. The nutritional value of the Se-containing proteins in Se-enriched soybeans
will be an interesting topic for the future studies. |
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