茚三酮为显色剂分光光度法测定氨苄西林钠

采用分光光度法研究了氨苄西林钠与茚三酮间的显色反应。 确定了反应条件，建立了一种快速、简便测定氨苄西林钠的分光光度法。 实验表明，氨苄西林钠与茚三酮在pH=5.4的水溶液中，沸水浴1 h可以反应完全，产物在λmax=564 nm下，表观摩尔吸光系数为3.72×103 L/(mol·cm)。 氨苄西林钠质量浓度在10~90 mg/L范围内服从比耳定律, 相关系数为0.9981，当氨苄西林钠浓度为40 mg/L时，测定结果的相对标准偏差为1.5%(n=6)，回收率在99.0%以上。

Spectrophotometric Determination of Ampicillin Sodium Using Ninhydrin as Chromogenic Agents

The reaction between ampicillin sodium and ninhydrin was investigated by spectrophotometry.A simple and rapid method for the determination of ampicillin sodium has been optimized.The apparent molar absorptivity of complex is 3.72×103 L/(mol·cm) at 564 nm.Beer’s law could be followed in the range of 10～90 mg/L of ampicillin sodium with correlation coefficient of 0.998 1.The relative standard deviation is 1.5%(n=6) and the recovery was over 99.0%.