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Capillary zone electrophoretic separation and determination of imidazolic antifungal drugs
Authors:Arranz A  Echevarría C  Moreda J M  Cid A  Arranz J F
Institution:Departamento de Química Analítica, Facultad de Farmacia, Universidad del País Vasco/EHU, Vitoria, Spain. qaparvaj@vc.ehu.es
Abstract:Capillary zone electrophoresis (CZE) was adapted to the simultaneous determination of a mixture of three imidazolic antifungal drugs. Separation was achieved by using a fused-silica capillary column with an acetic acid-Tris buffer at pH 5.18 and UV detection at 196 nm. Several electrophoretic parameters were investigated: pH and buffer concentration, applied voltage, temperature and injection conditions. The optimized CZE method was applied to the individual determination of ketoconazole, clotrimazole and econazole in pharmaceutical forms, after a previous single extraction step in methanol, with recoveries of 98.00, 99.96 and 99.58% respectively. The antifungal drugs can be determined at a concentration level lower than 1.0 x 10(-7) M.
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