毛细管电泳和电感耦合等离子体质谱接口技术进展

毛细管电泳与电感耦合等离子体质谱联用 (CE ICP MS)具有分离效率高、灵敏度高、分辨率高、样品用量少和检测限低等优点。已广泛应用于环境、生物等样品的元素形态分析。毛细管电泳与ICP MS雾化器之间的接口是CE ICP MS联用技术的关键。本文综述了影响接口性能的重要因素 ,包括毛细管出口端的电流导通、雾化器的设计和流速匹配等 ;讨论了保证毛细管内电流导通并稳定四种方法 :同心包层液导电、不锈钢组件导电、银箔导电及铂丝导电等 ;综述并比较了同心气动雾化器、微同心雾化器、高效雾化器、新接口技术、超声雾化器、直接注射雾化器六种类型的雾化器的结构和性能。引用文献 35篇。

Progress in Techniques of Interfacing Capillary Electrophoresis with Inductively Coupled Plasma Mass Spectrometry

The coupling of capillary electrophoresis (CE) with inductively coupled plasma mass spectrometry (ICP-MS) has demonstrated multiple advantages, including high separation efficiency, sensitivity and resolution, as well as low sample consumption and detection limit. The technique has been widely used for the speciation of environmental and biological samples. The interface between CE and the nebulizing system of ICP-MS plays a key role in the hyphenation of CE and ICP-MS. The most important factors in the interface design were reviewed in this paper, including electrical connections, nebulizer designs and flow compensation in the nebulizer system. Methods for achieving stable electrical current within the capillary were discussed, including the employment of concentric sheath flow, stainless steel components, platinum wire and silver paint. The structures and performance of various nebulizer designs were reviewed and compared, including the concentric pneumatic nebulizer (CPN), micro concentric nebulizer (MCN), high efficiency nebulizer (HEN), ultrasonic nebulizer (USN) and direct injection nebulizer (DIN). To eliminate nebulizer suction, four compensations systems were suggested, including applying a negative pressure at the inlet of the capillary, putting a sol-gel frit at the outlet of the capillary, pumping an appropriate make-up flow to the interface and adjusting the height of the make-up flow. 35 papers were cited.