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Capillary array isoelectric focusing with laser-induced fluorescence detection: milli-pH unit resolution and yoctomole mass detection limits in a 32-channel system |
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| Authors: | Oluwatosin O Dada Lauren M Ramsay Jane A Dickerson Nathan Cermak Rong Jiang Cuiru Zhu Norman J Dovichi |
| Institution: | (1) Chemistry Department, University of Washington, Seattle, WA 98195-1700, USA;(2) Chemistry Department, University of Alberta, Edmonton, AB, T6G 2M7, Canada;(3) Present address: Midwest Energy Group, INC, Carbondale, IL 62903, USA;(4) Present address: Great Southern Laboratories, Houston, TX 77099, USA; |
| Abstract: | We report a multiplexed capillary electrophoresis system employing an array of 32 capillaries with a micromachined sheath-flow cuvette as the detection chamber. The sample streams were simultaneously excited with a 473-nm laser beam, and the fluorescence emission was imaged on a CCD camera with a pair of doublet achromat lens. The instrument produced mass detection limits of 380 ± 120 yoctomoles for fluorescein in zone electrophoresis. Capillary isoelectric focusing of fluorescent standards produced peaks with an average width of 0.0029 ± 0.0008 pH. Capillary coating stability limits the reproducibility of the analysis. |
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