Localized In Vivo Activation of a Photoactivatable Doxorubicin Prodrug in Deep Tumor Tissue |
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Authors: | Stuart Ibsen Eran Zahavy Wolf Wrasidlo Tomoko Hayashi John Norton Yongxuan Su Stephen Adams Sadik Esener |
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Affiliation: | 1. Department of Bioengineering, Moores Cancer Center, University of California San Diego, , La Jolla, CA;2. Department of Infectious Disease, Israel Institute for Biological Research, , Ness‐Ziona, Israel;3. Moores Cancer Center, University of California at San Diego, , La Jolla, CA;4. Department of Chemistry and Biochemistry, University of California at San Diego, , La Jolla, CA;5. Department of Pharmacology, University of California at San Diego, , La Jolla, CA;6. Department of Nanoengineering, University of California at San Diego, , La Jolla, CA |
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Abstract: | Sparing sensitive healthy tissue from chemotherapy exposure is a critical challenge in the treatment of cancer. The work described here demonstrates the localized in vivo photoactivation of a new chemotherapy prodrug of doxorubicin (DOX). The DOX prodrug (DOX‐PCB) was 200 times less toxic than DOX and was designed to release pure DOX when exposed to 365 nm light. This wavelength was chosen because it had good tissue penetration through a 1 cm diameter tumor, but had very low skin penetration, due to melanin absorption, preventing uncontrolled activation from outside sources. The light was delivered specifically to the tumor tissue using a specialized fiber‐optic LED system. Pharmacokinetic studies showed that DOX‐PCB had an α circulation half‐life of 10 min which was comparable to that of DOX at 20 min. DOX‐PCB demonstrated resistance to metabolic cleavage ensuring that exposure to 365 nm light was the main mode of in vivo activation. Tissue extractions from tumors exposed to 365 nm light in vivo showed the presence of DOX‐PCB as well as activated DOX. The exposed tumors had six times more DOX concentration than nearby unexposed control tumors. This in vivo proof of concept demonstrates the first preferential activation of a photocleavable prodrug in deep tumor tissue. |
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