Sensitive electrochemical detection of the hydroxyl radical using enzyme-catalyzed redox cycling |
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Authors: | Tatsumi Hirosuke Osaku Naoya |
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Affiliation: | International Young Researchers Empowerment Center, Shinshu University, Matsumoto, Nagano, Japan. tatsumi@shinshu-u.ac.jp |
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Abstract: | Enzyme-catalyzed signal amplification was introduced to the electrochemical detection of the OH radical. In the presence of phenol as a trapping agent, glucose as a substrate, and pyrroloquinoline quinone-containing glucose dehydrogenase (PQQ-GDH) as a catalyst, the current signal for the trapping adducts (catechol and hydroquinone) produced by the hydroxylation of phenol could be amplified and detected sensitively. The limit of detection (S/N = 3) for catechol was 8 nM. The trapping efficiency of phenol was also estimated. |
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