| Abstract: | A simple protocol based on polymerization reactions has been developed for the preparation of high‐loading polymer supports, useful for large‐scale synthesis of oligonucleotides. Polymer supports of different pore sizes have been employed in the present investigation to improve the functional‐group density on them. A ten‐ to twelvefold increase in the loading of the functional groups, after the polymerization reaction, has been observed. The support was then used in the subsequent reaction to attach the leader nucleoside to obtain fully functionalized supports 6a – 6c by oligonucleotide synthesis in an automated DNA synthesizer. The aminoalkylated‐supports 5a – 5c were directly employed for the synthesis of oligonucleotide 3′‐phosphates. The oligonucleotides and oligonucleotide 3′‐phosphates synthesized on these supports were compared with the corresponding standard oligomers with respect to their retention time on HPLC. These were further characterized on MALDI‐TOF mass spectrometry. |
