Rapid determination of acetaminophen and p-aminophenol in pharmaceutical formulations using miniaturized capillary electrophoresis with amperometric detection |
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Authors: | Chu Qingcui Jiang Lianmei Tian Xiuhui Ye Jiannong |
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Institution: | Department of Chemistry, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, PR China |
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Abstract: | Capability of fast analysis of a novel miniaturized capillary electrophoresis with carbon disk electrode amperometric detection (mini-CE-AD) system was demonstrated by determining acetaminophen and p-aminophenol in dosage forms. Factors influencing the separation and detection processes were examined and optimized. Under the optimum conditions, the end-capillary 300 μm carbon disc electrode amperometric detector offered favorable signal-to-noise characteristics at a relatively low potential (+600 mV versus Ag/AgCl) for detecting acetaminophen and p-aminophenol. Two analytes can been separated within 150 s in a 8.5 cm length capillary at a separation voltage of 2000 V using a Na2B4O7-KH2PO4 running buffer (pH 7.2). Acetaminophen and p-aminophenol could be detected down to the 1.4 × 10−6-5.9 × 10−7 mol L−1 level with linearity up to the 1.0 × 10−3 mol L−1 level examined. The inter-day repeatability for analytes in peak current (R.S.D. ≤ 2.3%) and migration times (R.S.D. ≤ 1.3%) were excellent. The proposed mini-CE-AD system should find a wide range of analytical applications in pharmaceutical formulations as an alternative to conventional CE and μ-CE. |
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Keywords: | Capillary electrophoresis Amperometric detection Miniaturization Acetaminophen p-Aminophenol |
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