Rapid determination of beta-aminoisobutyric acid by reversed-phase high-performance liquid chromatography |
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| Authors: | O Ladrón de Guevara C Cortinas de Nava P Padilla J Espinosa M Cebrian L García |
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| Affiliation: | Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico, D.F. |
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| Abstract: | For the determination of beta-aminoisobutyric acid (BAIBA) in urine samples in which the beta-alanine concentrations are higher than those of BAIBA, the resolution between these two amino acids, separated by reversed-phase liquid chromatography on an octadecylsilane column, was optimized. The chromatographic analysis included precolumn derivatization of amino acids with o-phthalaldehyde, followed by a 15-min isocratic elution and detection at 340 nm. Because of its simplicity, this method should be useful for monitoring urinary excretion of BAIBA. |
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