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Determination of Dextromethorphan and Dextrorphan in Human Urine by High Performance Liquid Chromatography for Pharmacogenetic Investigations |
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| Authors: | J Flores-Pérez C Flores-Pérez H Juárez-Olguín I Lares-Asseff M Sosa-Macías |
| Institution: | (1) Laboratorio de Farmacología, Instituto Nacional de Pediatría, Avenida Imán No.1, 3er piso Colonia Cuicuilco, CP 04530 México D.F., México;(2) Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de, México;(3) Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Durango, Instituto Politécnico Nacional, México |
| Abstract: | The aim of the present study was to develop a sensitive method to measure dextromethorphan and dextrorphan in urine by HPLC to support pharmacogenetic studies in ethnic groups. Linearity was assessed in the range: 0.015–10  g mL–1 for dextromethorphan and 1-10 g mL–1 for dextrorphan. Inter and intra-day coefficients of variation were < 10%. Limits of detection and quantitation were 0.003 g mL–1 and 0.015 g mL–1 for dextromethorphan and 0.24 g mL–1 and 1.0 g mL–1 for dextrorphan, respectively. The method is reliable in helping determine the phenotype of Mexican ethnic groups using model drugs such as dextromethorphan. |
| Keywords: | Column liquid chromatography Pharmacogenetic studies Dextromethorphan and dextrorphan Urine samples Mexican ethnic groups |
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