A Quantitative Spectrometric Assay for the Determination of Solution Concentration of para-sulphonato-calix[n]arenes and Their Derivatives

A spectrometric assay for the determination of concentration of para-sulphonato-calixn]arenes and their derivatives has been developed using dimethylmethylene blue (DMMB) as a probe. Interaction with para-sulphonato-calixn]arenes leads to a metachromatic shift in the spectrum of DMMB with appearance of a peak at 536 nm and diminution of the spectral intensity of the peaks at 594 and 649 nm. The method shows good linearity in the concentration range 0–6 μg/ml for para-sulphonato-calixn]arenes. in final form: 27 December 2004