| 基本培养基中芳香族氨基酸和维生素对D-核糖生产的影响 |
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| 引用本文： | 吴琳,李志敏,叶勤.基本培养基中芳香族氨基酸和维生素对D-核糖生产的影响[J].华东理工大学学报(自然科学版),2009,35(5). |
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| 作者姓名： | 吴琳 李志敏 叶勤 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 摘 要： | 考察了芳香族氨基酸和维生素对转酮醇酶缺失的枯草芽孢杆菌生长以及D-核糖生产的影响.实验结果表明:L-酪氨酸、L-色氨酸、L-苯丙氨酸、生物素、烟酸、吡哆醛的添加能改善菌体生长,提高D-核糖产量,特别是烟酸作用尤其明显.在单因素实验基础上,采用响应面分析方法对培养基中的维生素添加量进行了优化,确定最佳培养基组成.在此优化培养基中D-核糖质量浓度达到(23.4±0.39)g/L,与预测结果一致,是不添加维生素时核糖产量的2.31倍,D-核糖对葡萄糖的得率达到0.353 mol/mol.
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| 关 键 词： | 枯草芽孢杆菌 D-核糖 烟酸 吡哆醛 |
Effect of Aromatic Amino Acids and Vitamins on D-Ribose Production and Optimization of Minimal Medium |
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| WU Lin,LI Zhi-min,YE Qin.Effect of Aromatic Amino Acids and Vitamins on D-Ribose Production and Optimization of Minimal Medium[J].Journal of East China University of Science and Technology,2009,35(5). |
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| Authors: | WU Lin LI Zhi-min YE Qin |
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| Institution: | State Key Laboratory of Bioreactor Engineering;East China University of Science and Technology;Shanghai 200237;China |
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| Abstract: | In this study,effects of aromatic amino acids and vitamins on the growth of transketolase-deficient Bacillus subtilis and D-ribose biosynthesis were studied in chemically defined media.It was found that aromatic amino acids,biotin,pyridoxine and nicotinic acid promoted cell growth,and nicotinic acid enhanced D-ribose production significantly.The response surface methodology(RSM) was applied to optimize the concentration of vitamins in the minimal medium.Under optimal condition,the production of D-ribose was... |
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| Keywords: | Bacillus subtilis D-ribose nicotinic acid pyridoxine |
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