| 键合型手性固定相拆分麻醉药JM-1232 |
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| 引用本文： | 何晏霞,张平,高玲,杨孟昌,田雪梅,刘敏,邓奇,唐梨.键合型手性固定相拆分麻醉药JM-1232[J].分析试验室,2021,40(2):213-216. |
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| 作者姓名： | 何晏霞 张平 高玲 杨孟昌 田雪梅 刘敏 邓奇 唐梨 |
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| 作者单位： | 四川省人民医院,成都610076;四川省人民医院,成都610076;四川省人民医院,成都610076;四川省人民医院,成都610076;四川省人民医院,成都610076;四川省人民医院,成都610076;四川省人民医院,成都610076;四川省人民医院,成都610076 |
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| 摘 要： | 使用Chiralpak IA色谱柱,实现了麻醉药JM-1232的对映体分离,并进一步考优化了流动相组成、柱温和流速等条件。得到最优色谱条件:流动相为正己烷:异丙醇=65:35(V/V)、柱温30℃、流速1.0 mL/min,分离度达2.31。该方法显示出JM-1232对映体的足够分离度。
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| 关 键 词： | 高效液相色谱 键合型多糖类手性固定相 JM-1232 对映体拆分 |
Enantioseparation of narcotic JM-1232 on immobilized chiral stationary phases |
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| HE Yanxia,ZHANG Ping,GAO Ling,YANG Mengchang,TIAN Xuemei,LIU Min,DENG Qi,TANG Li.Enantioseparation of narcotic JM-1232 on immobilized chiral stationary phases[J].Chinese Journal of Analysis Laboratory,2021,40(2):213-216. |
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| Authors: | HE Yanxia ZHANG Ping GAO Ling YANG Mengchang TIAN Xuemei LIU Min DENG Qi TANG Li |
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| Institution: | (Sichuan Provincial People’s Hospital,Chengdu 610072) |
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| Abstract: | Chromatographic model was employed for enantioseparation of JM-1232 on the Chiralpak IA column.The effects of chromatography conditions,such as mobile phase,temperature and flow rate to chiral recognition,had been investigated.The results showed that a good separation was obtained at 30℃by using hexane-isopropyl alcohol(65:35,V/V)as the mobile phase with a flow rate of 1.0 mL/min.The resolution was 2.31.The method showed adequate resolution for JM-1232 enantiomers and was validated for linearity,precision,accuracy,selectivity and robustness. |
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| Keywords: | high performance liquid chromatography immobilized polysaccharide-based chiral stationary phases JM-1232 enantioseparation |
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