Radio-gas-chromatographische Kontrolle des 14C-Acetateinbaus in die Phosphatidfettsäuren der Sojabohnen

Germinating soybeans (glycine max.L.) can be employed to give a good yield of labelled phosphatides. The incorporation of ¹⁴C-acetate into fatty acids of the phosphatides, which under optimal conditions (35°C, 3000 Lux) is about 15%, was radio-gas-chromatographically controlled. It was found that exposure to light causes an increase in the formation of unsaturated fatty acids. Under the influence of a pure oxygen atmosphere at 16°C the phosphatides obtained have about 50% of the ¹⁴C-radioactivity in the oleic acid and 20% in linolic acid. This gives the preconditions necessary to obtain natural phosphatides with unequal distribution of radioactivity in the saturated and unsaturated fatty acids.