Direct imaging of aligned neurofilament networks assembled using in situ dialysis in microchannels |
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Authors: | Hesse H C Beck R Ding C Jones J B Deek J MacDonald N C Li Y Safinya C R |
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Affiliation: | Materials Department, University of California, Santa Barbara, California 93106, USA. |
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Abstract: | We report a technique to produce aligned neurofilament networks for direct imaging and diffraction studies using in situ dialysis in a microfluidic device. The alignment is achieved by assembling neurofilaments from protein subunits confined within microchannels. Resulting network structure was probed by polarized optical microscopy and atomic force microscopy, which confirmed a high degree of protein alignment inside the microchannels. This technique can be expanded to facilitate structural studies of a wide range of filamentous proteins and their hierarchical assemblies under varying assembly conditions. |
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