A microsystem of low-voltage-driven electrophoresis on microchip with array electrode pairs for the separation of amino acids |
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Authors: | Yi Xu Xiaoguo Hu Jing Liang Jianxin Sun Wenwen Gu Tianming Zhao and Zhiyu Wen |
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Institution: | (1) Key Disciplines Lab of Novel Micro–Nano Devices and System Technology, Chongqing University, Chongqing, 400030, China;(2) International R&D Center of Micro–Nano Systems and New Materials Technology, Chongqing University, Chongqing, 400030, China;(3) Microsystem Research Center, Chongqing University, Chongqing, 400030, China;(4) Chemistry and Chemical Engineering College, Chongqing University, Chongqing, 400030, China |
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Abstract: | In this paper, a new approach for the separation of amino acids on the electrophoresis chip-based low-voltage-driven electrophoresis
was reported in detail. This low-voltage-driven electrophoresis process could be realized by powering directly the arrayed
electrode pairs with low direct current (DC) voltage to generate a moving electric field along the separation microchannel,
which could maintain enough electric field strength for electrophoresis. The proposed microfluidic electrophoresis chip was
bonded directly with silicon-on-insulator (SOI) substrate and polydimethylsiloxane (PDMS) cover plate at room temperature.
The microfluidic channels and the arrayed electrodes were etched on SOI wafer by silicon microelectromechanical system technology.
A specially integrated circuit was proposed to power a 30–60-V DC voltage to particular sets of these electrode pairs in a
controlled sequence such that the moving electric field could be formed, and the low-voltage-driven electrophoresis could
be realized in the microchannel. In the experiments, with 10−4 mol/L phenylalanine and lysine as analytes, the separation of amino acids on the low-voltage-driven electrophoresis microchip
was conducted by homemade integrated control circuit; a method for separating amino acids was well established. It was also
shown that the phenylalanine and lysine mixture was effectively separated in less than 7 min and with a resolution of 2.0.
To the best of our knowledge, the low-voltage-driven microchip electrophoresis device could be of potential prospective in
the fields of integrated and miniaturized biochemical analysis system.
This work was financially supported by the Chinese National Science and Technology Committee “863 PLAN” (2006AA04Z345) and
“International Cooperation Plan” (2006DFA13510) also by the Nature and Science Fund (no. 20675089) and (90307015) of Chinese
National Educational Committee. |
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Keywords: | Electrophoresis driven by low-voltage on microchip Microelectrode array Integrated circuit Separation of amino acids Microelectromechanical systems technology |
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