Single Molecule Genotyping by TIRF Microscopy |
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| Authors: | Steffen Rüttinger Baptiste Lamarre Alex E Knight |
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| Institution: | (1) Biomedical Optics Department, Physikalisch-Technische Bundesanstalt, Berlin, Germany;(2) Biotechnology Group, National Physical Laboratory, Hampton Road, Teddington, Middlesex, TW11 0LW, UK |
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| Abstract: | As part of a programme to develop a metrological framework for single molecule measurements in biology, we have investigated
the applications of single molecule imaging to genomics. Specifically, we have developed a technique for measuring the frequencies
of single nucleotide polymorphisms (SNPs) in complex or pooled samples of DNA. We believe that this technique has applications
to statistical genotyping—the identification of correlations between SNP frequencies and particular phenotypes—and other areas
where it is desirable to track the frequencies of SNPs in complex DNA populations. |
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| Keywords: | Single molecule imaging Total internal reflection fluorescence microscopy Statistical genotyping Single nucleotide polymorphism |
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