| 光固定EGF对CHO-K1细胞生长的促进 |
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| 引用本文： | 关燕清.光固定EGF对CHO-K1细胞生长的促进[J].离子交换与吸附,1999(1). |
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| 作者姓名： | 关燕清 |
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| 作者单位： | 华南师范大学生物技术研究所!广州，510631 |
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| 摘 要： | 用活化的4-叠氮苯甲酸与上皮细胞生长因子EGF反应，合成了具有光反应性的EGF。将这种EGF涂覆在组织培养聚苯乙烯基板上，通过紫外光照射，EGF与聚苯乙烯基板通过共价键连接。在此材料上进行中国仓鼠卵巢细胞CHO－K1的无血清培养，并对细胞生长活性进行研究。
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| 关 键 词： | 上皮细胞生长因子 EGF 光固定 聚苯乙烯基板 中国仓鼠卵巢 细胞CHO-K1 无血清培养 细胞生长活性 |
CHINESE HAMSTER OVARY CELLS GROWTH ON THE PHOTO-IMMOBILIZATION OF EPIDERMAL GROWTH FACTOR POLYSTYRENE PLATE |
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| Guan Yanqing.CHINESE HAMSTER OVARY CELLS GROWTH ON THE PHOTO-IMMOBILIZATION OF EPIDERMAL GROWTH FACTOR POLYSTYRENE PLATE[J].Ion Exchange and Adsorption,1999(1). |
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| Authors: | Guan Yanqing |
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| Abstract: | Photo-reactive Epidermal Growth Factor (EGF) was synthesized by coupling EGF with (N-(4-azidobenzoyloxy)succinimid and coated onto the 24-well cell culture polystyrene plate,then Ultraviolet light was irradiated on the coated surface for chemical fixation of EGF via the conversion of the phenylaaldo group to the highly reactive phenyl-nitrene which spontaneously formed covalent bonds with neighboring hydro-carbons. on these EGF-immobilized polystyrene plates, the Chinese harnster ovary (CHO-K1) cell were cultured under the serum-free conditions, and the cell groWth activity were investigated. |
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| Keywords: | EGF Photo-immobilization Polystyrene plate Chinese Hamster Ovary (CHO-K1) cell Serum-free culture Cell growth activity |
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