14CO2 labeling : a reliable technique for rapid measurement of total root exudation capacity and vascular sap flow in crop plants |
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Authors: | Bhupinder Singh Sumedha Ahuja Renu Pandey RK Singhal |
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Institution: | 1. Nuclear Research Laboratory, Indian Agricultural Research Institute, New Delhi, 110012, India 2. Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110012, India 3. Analytical Spectroscopy Section, Analytical Chemistry Division, Mod Lab, BARC, Trombay, Mumbai, 400085, India
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Abstract: | Root release of organic compounds and rate of the vascular sap flow are important for understanding the nutrient and the source-sink dynamics in plants, however, their determination is procedurally cumbersome and time consuming. We report here a simple method involving 14C labeling for rapid and reliable measurement of root exudates and vascular sap flow rate in a variable groundnut population developed through seed gamma irradiation using a cobalt source (60Co). An experimental hypothesis that a higher 14C level in the vascular sap would indicate a higher root release of carbon by the roots into the rhizosphere was verified. |
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