金属硫蛋白溶液聚合状态的研究

通过动态光散射实验首次证明了兔肝金属硫蛋白亚型 和 在不同的缓冲体系中不仅仅是以二聚体的形式 ,而且是以多种聚合形式存在1] .深入考察金属硫蛋白在溶液中的聚合形式对于研究金属硫蛋白的结构和功能都具有非常重要的意义 .我们通过计算机模拟 1] 比较系统地研究了各种因素对金属硫蛋白聚合的影响 .结果表明 ,在溶液聚合的分子识别过程中可由多种因素共同调控金属硫蛋白 ,这些因素主要包括静电相互作用、疏水性相互作用和溶液中阴阳离子等 .通过这些因素的综合分析 ,首次对兔肝金属硫蛋白在溶液中多种聚合形式的形成机制及相应的分子…

Studies on the Metallothionein Polymerization in Different Buffers

In this paper, the naturally occurred polymerization for rabbit metallothionein monomers has been studied in different milieu, while leaves out the aggregation caused by the oxidation of cysteine, since the latter circumstance was the result of purification lapsus. After the purification of metallothionein monomers, dynamic light scattering technique is used to detect the polymerized states of rabbit liver metallothionein Ⅰ and Ⅱ in different buffers, which is the first systematical detection of polymerized states of MTs in solutions. The effects of different factors contributing to methothionein aggregations have been investigated by computational simulations. The calculation results showed that the metallothionein polymerization would be regulated by many factors including electrostatic interactions, hydrophobic interactions and different ions in buffers. A molecular recognition mechanism of MTs' polymerization in solutions is proposed on the bases of experimental results and theoretical calculations.