Within the cell: analytical techniques for subcellular analysis |
| |
| Authors: | Karen?J?Olson Hossein?Ahmadzadeh Email author" target="_blank">Edgar?A?ArriagaEmail author |
| |
| Institution: | (1) Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, MN 55455, USA;(2) Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN 55455, USA |
| |
| Abstract: | This review covers recent developments in the preparation, manipulation, and analyses of subcellular environments. In particular, it highlights approaches for (1) separation and detection of individual organelles, (2) preparation of ultra-pure organelle fractions, and (3) utilization of novel labeling strategies. These approaches, based on innovative technologies such as microfluidics, immunoisolation, mass spectrometry and electrophoresis, suggest that subcellular analyses will soon become as commonplace as single cell and bulk cellular assays. |
| |
| Keywords: | Subcellular analysis Organelle Electrophoresis Flow cytometry |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
