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Differential pulse polarographic behaviors of p-nitrosodimethylaniline: Application for microdetermination of NADH
Authors:ST Sulaiman  MM Najeeb
Institution:1. Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq;2. Department of Medical Biochemistry, College of Medicine, University of Mosul, Mosul, Iraq
Abstract:The differential pulse polarographic behavior of p-nitrosodimethylaniline has been investigated in Britton-Robinson buffer and phosphate buffer. The peak obtained at pH 8.0 is recommended for the trace determination of this compound, with an experimental detection limit of 18 ppb (1.2 × 10?7M) in simple aqueous solution. The method is also applied for the indirect microdetermination of NADH. The experimental detection limit of NADH is shown to be 1.05 × 10?6M.
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