Electrochemiluminescence biosensor for carcinoembryonic antigen detection based on Au-Ag/g-C3N4 nanocomposites |
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Institution: | 1. Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu 476000, PR China;2. Central Lab at Zhengzhou First People Hospital, Zhengzhou First People Hospital, Zhengzhou 450000, PR China |
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Abstract: | Herein, an electrochemiluminescence (ECL) aptasensor for carcinoembryonic antigen (CEA) detection was developed based on Au-Ag/g-C3N4 nanocomposites (NCs), which were synthesized by decorating graphitic carbon nitride (g-C3N4) nanosheets with alloy-structured Au-Ag bimetallic nanoparticles (NPs) via one-step in situ chemical reduction. As ECL sensing platform, Au-Ag/g-C3N4 NCs could significantly improve the ECL intensity of luminol due to the good conductivity of Au-Ag NPs, electrocatalytic activity for oxygen evolution reaction (OER) and the ability to adsorb luminol via π stacking interaction. In addition, it could load the thiol terminated aptamers of CEA via Au-S or Ag-S bonds. In the presence of CEA, the ECL response of the proposed biosensor decreased significantly due to the fact that the assembled protein layers hindered the electron transfer and the diffusion of ECL reactants toward the electrode surface. The proposed ECL sensor exhibited a good linear relationship with CEA in the range of 1.0–1.0 × 10?6 ng/mL with a detection limit of 8.9 × 10?7 ng/mL. The satisfactory results were obtained in the detection of CEA in human serum samples. |
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Keywords: | Electrochemiluminescence Carcinoembryonic antigen Aptasensor |
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