Metabolomic profile and in vitro evaluation of the cytotoxic activity of Asphodelus microcarpus against human malignant melanoma cells A375 |
| |
| Affiliation: | 1. Department of Natural Resources, Environmental Studies and Research Institute, University of Sadat City, Sadat City, Egypt;2. Department of Cytology & Histology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt;3. Animal Biotechnology Department Genetic Engineering and Biotechnology Institute (GEBRI), University of Sadat City, Sadat City, Egypt;4. Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 10691 Stockholm, Sweden;5. Pharmacognosy Group, Department of Pharmaceutical Biosciences, BMC, Uppsala University, Uppsala, Box 591, SE 751 24 Uppsala, Sweden;6. International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China;7. International Joint Research Laboratory of Intelligent Agriculture and Agri-products Processing, Jiangsu Education Department, Jiangsu University, China;8. Department of Pharmacognosy, Faculty of Pharmacy, University of Sadat City, Menoufia 32897, Egypt;9. Department of Chemistry, Faculty of Science, Menoufia University, Menoufia 32511, Egypt |
| |
| Abstract: | Melanoma is a huge worldwide health problem that must be handled more effectively with better therapeutic options. As a result, new treatment drugs are required to treat this condition. The goal of this study was to investigate the cytotoxic activity of the anthraquinone-rich fractions obtained from Asphodelus microcarpus against human melanoma cell A375. On these melanoma cell lines; the cytotoxicity of these fractions had never been studied before. Liquid chromatography linked to mass spectrometry (LC-MS-MS) and Nuclear Magnetic Resonance was used to determine the chemical profiles of these fractions. The cytotoxicity of the fractions studied was determined by measuring cell viability and calculating IC50 values. Both ethyl acetate (EtOAC) and the precipitate fractions (PPT) exhibited selective cytotoxicity on human melanoma A 375 cell line with IC50 values of 83 and 65 µg/mL, respectively. The antiproliferative properties of EtOAc fraction and PPT were supported by a noticeable decrease in cell numbers during the G2/M cell cycle arrest. Our findings suggest that the anthraquinone content of A. microcarpus tubers is responsible for its anti-proliferative and apoptotic properties and that further in vivo investigations should be conducted to establish the viability of using them to treat human melanomas. |
| |
| Keywords: | Anthraquinone Apoptosis Melanoma Metabolomic Cell cycle |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
