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Green synthesis of chitosan-stabilized silver-colloidal nanoparticles immobilized on white-silica-gel beads and the antibacterial activities in a simulated-air-filter
Affiliation:1. Graduate School of Mathematics and Applied Science, Universitas Syiah Kuala, Darussalam Banda Aceh 23111, Indonesia;2. Chemistry Department, FKIP, Universitas Syiah Kuala, Darussalam Banda Aceh 23111, Indonesia;3. Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Darussalam Banda Aceh 23111, Indonesia;4. Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Darussalam Banda Aceh 23111, Indonesia;5. Department of Microbiology, School of Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia;6. School of Chemical Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia
Abstract:This study explored the green synthesis and immobilization of colloidal silver nanoparticles (AgNPs) on a solid compatible support. Its antibacterial properties in reusable air filters are also discussed. The chitosan stabilized colloidal AgNPs (chi-AgNPs) were prepared using visible light irradiation in methanol. The UV–Vis, FTIR spectra, and TEM confirmed the chi-AgNPs formation. The immobilization technique of chi-AgNPs on the surface of white-silica-gel beads, which was previously coated chitosan (chi-SiG), was effective. The immobilized silver particles (AgNPs-[chi-SiG]) were solid, stable, dispersed, and nano-size. Both AgNPs-[chi-SiG] and chi-SiG exhibited antibacterial properties and prevented the growth of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria in agar media. Air filter containing the AgNPs-[chi-SiG] showed high antibacterial activity against Bacillus subtilis in the air.
Keywords:Immobilization  Silver nanoparticles  Chitosan  Antibacterial  Air filter
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