A flow-injection system using immobilized peroxidase and chromogenic reagents for possible determination of hydrogen peroxide

Summary A flow injection system with a reactor containing peroxidase immobilized on porous glass was used for the determination of hydrogen peroxide. A number of chromogenic reagents were studied in combination with different immobilization methods, in particular with regard to adsorption of the coloured product on the enzyme support. A f. i. a. manifold had a throughout of 210 samples h^–1 and a detection limit of 0.1 M H₂O₂ and could be used for at least 17000 determinations with the same enzyme reactor. Systems with higher sensitivity or higher sample throughput are also described.