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Glucose Biosensor Using Glucose Oxidase Immobilized in Polyaniline
Authors:A H Parente  E T A Marques  W M Azevedo  F B Diniz  E H M Melo  J L Lima Filho
Institution:(1) Departamento de Bioquimica, CCB, Laboratorio de Imunopatologia Keizo Asami-LIKA, Brazil;(2) Departamento de Quimica Fundamental, CCEN, Universidade Federal de Pernambuco-Cidade Universitaria, CEP 50730 Recife, PE, Brazil
Abstract:A biosensor for glucose utilizing kinetics of glucose oxidase (EC 1.1.3.4.) was developed. The enzyme was immobilized on polyaniline by covalent bonding, using glutaraldehyde as a bifunctional agent. The system showed a linear response up to 2.2 mM of glucose with a response time of 2.5–4.0 min. In addition, the immobilized enzyme had a higher activity between pH 6.5 and 7.5. The system retained 50% of its activity after 30 d of daily use. The optical absorption spectra of the polyaniline/glucose oxidase electrode after glucose had been added to the buffer solution showed that the absorption band around 800 nm had changed considerably when glucose was allowed to react with the electrode. This optical variation makes polyaniline a very promising polymer for use as a support in optical sensor for clinical application.
Keywords:Glucose oxidase  polyaniline  biosensor  oxygen  electrode
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