| 碱性品红修饰磁性纳米粒子的制备及在分枝杆菌分离中的应用 |
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| 引用本文： | 察冬梅,韩朝晖,马甜,李步海,刘国诠,祝伟.碱性品红修饰磁性纳米粒子的制备及在分枝杆菌分离中的应用[J].高等学校化学学报,2013,34(4):760-762. |
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| 作者姓名： | 察冬梅 韩朝晖 马甜 李步海 刘国诠 祝伟 |
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| 作者单位： | 1. 中南民族大学化学与材料科学学院分析化学国家民委重点实验室, 武汉 430074;
2. 中国科学院化学研究所, 北京 100190;
3. 中南民族大学生命科学学院, 武汉 430074 |
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| 基金项目： | 湖北省教育厅指导性科研计划项目(批准号:B20110809)和中南民族大学学术团队科研基金项目(批准号:XTZ09005)资助. |
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| 摘 要： | 痰或其它各种体液内结核分枝杆菌(MTB)阳性是诊断结核病(TB)的金标准.结核硬脂酸(TBSA)是鉴定分枝杆菌与其它非分枝杆菌的特征组分,可用于TB的诊断1,2]。
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| 关 键 词： | 磁性微球 碱性品红 细菌分离 分枝杆菌 |
| 收稿时间： | 2012-10-05 |
Preparation of Basic Fuchsin Modified Magnetic Microspheres and Its Application in Isolation of Mycobacterium |
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| CHA Dong-Mei,HAN Zhao-Hui,MA Tian,LI Bu-Hai,LIU Guo-Quan,ZHU Wei.Preparation of Basic Fuchsin Modified Magnetic Microspheres and Its Application in Isolation of Mycobacterium[J].Chemical Research In Chinese Universities,2013,34(4):760-762. |
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| Authors: | CHA Dong-Mei HAN Zhao-Hui MA Tian LI Bu-Hai LIU Guo-Quan ZHU Wei |
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| Institution: | 1. Key Laboratory for Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Material Science, South-Central University for Nationalities, Wuhan 430074, China;
2. Institute of Chemistry, The Chinese Academy of Sciences, Beijing 100190, China;
3. College of Life Science, South-Central University for Nationalities, Wuhan 430074, China |
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| Abstract: | Two kinds of methods were used to prepare basic fuchsin(BF) modified magnetic nanoparticles. The obtained BF modified nanoparticles(MNP-1 and MNP-2) were characterized using transmission electron microscope(TEM) and Fourier transformation infrared(FTIR) spectroscopy before used to adsorb mycobacterium from Staphylococcus aureus and Escherichia coli(E. coli). The effects of pH, adsorption time, concentration of bacteria, temperature, ionic strength and adsorbent dosage on the adsorption of bacterium were studied. The results show that both MNP-1 and MNP-2 have good selectivity for mycobacterium, and can be easily separated by external magnetic field. Under the optimized conditions, the highest adsorption rate for mycobacteria were 65%(MNP-1) and 85%(MNP-2), while only 20% of Staphylococcus aureus and 10% of E. coli can be adsorbed under the same conditions. The adsorption capacity of MNP-1 and MNP-2 for mycobacteria was 1.48?010 and 3.25?010 cfu/g, respectively. |
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| Keywords: | Magnetic microspheres Basic fuchsin Bacterium separation Mycobacterium |
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