光波导共振技术结合比色法检测葡萄糖溶液的浓度

利用比色法结合双面金属包覆波导构建了一种高灵敏度的光波导振荡场传感器. 将葡萄糖溶液与氧化酶试剂混合, 使葡萄糖溶液显色并注入波导结构, 采用波长为650 nm的激光入射于波导表面, 经角度扫描产生衰减全反射(ATR)吸收峰, 通过ATR吸收峰的极小值来检测葡萄糖溶液的浓度变化. 实验结果表明, 该技术对葡萄糖溶液浓度的分辨率达到111 nmol/mL, 其灵敏度高于分光光度计.

Detection of the Concentration of Glucose Solution Using Optical Waveguide Resonance Technology Combined with Colorimetry

Glucose is a major component of the carbohydrates in the bodies of animals and plants, and it is very important to determine its quantitative in many areas. In this work, the colorimetric method combined with a double-sided metal coated waveguide was used to constitute a high sensitive oscillation field optical waveguide sensor. The glucose solution was colored by mixing with the oxidase reagent and then was injected into the waveguide. The attenuated total reflection(ATR) absorption peaks could be obtained through angle scanning, with the 650 nm laser beam incidenting on the surface of the waveguide. And the concentration changes of the glucose solution could be detected according to the minimum value of the absorption peaks. The results show that the resolution to the concentration of solution of this method can reach to 111 nmol/mL.