| 氯过氧化物酶修饰电极用于岩白菜素的电催化氯化 |
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| 引用本文： | 王珂,吴霞琴,李琳,田海涛,陆中庆. 氯过氧化物酶修饰电极用于岩白菜素的电催化氯化[J]. 高等学校化学学报, 2013, 34(7): 1739-1742. DOI: 10.7503/cjcu20120965 |
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| 作者姓名： | 王珂 吴霞琴 李琳 田海涛 陆中庆 |
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| 作者单位： | 上海师范大学生命与环境科学学院, 上海 200234 |
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| 基金项目： | 国家自然科学基金(批准号: 20973114)资助. |
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| 摘 要： | 将氯过氧化物酶(CPO)与双十二烷基二甲基溴化铵(DDAB)混合后滴涂于玻碳(GC)电极表面, 制得CPO-DDAB/GC修饰电极. 循环伏安结果显示, 固定在电极表面的CPO可与电极之间发生直接的电子传递作用. 利用高效液相色谱-质谱联用技术对反应产物进行表征, 结果显示, 以该CPO修饰电极为工作电极, 在氧气饱和的氯化钾-岩白菜素溶液中, 利用CPO催化氧还原生成的过氧化氢可进一步驱动CPO对岩白菜素的催化氯化反应. 经估算总转化数(TTN)达到13600, 即1 mol CPO可催化13600 mol岩白菜素.
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| 关 键 词： | 氯过氧化物酶 修饰电极 岩白菜素 生物酶催化 电催化 氯化 |
| 收稿时间： | 2012-10-25 |
Electrochemical Catalytic Chlorination of Bergenin by Chloroperoxidase Modified Electrode |
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| WANG Ke,WU Xia-Qin,LI Lin,TIAN Hai-Tao,LU Zhong-Qing. Electrochemical Catalytic Chlorination of Bergenin by Chloroperoxidase Modified Electrode[J]. Chemical Research In Chinese Universities, 2013, 34(7): 1739-1742. DOI: 10.7503/cjcu20120965 |
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| Authors: | WANG Ke WU Xia-Qin LI Lin TIAN Hai-Tao LU Zhong-Qing |
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| Affiliation: | College of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China |
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| Abstract: | A chloroperoxidase(CPO) modified electrode was fabricated by drop-coating the mixture solution of CPO and didodecyldimethylammonium bromide(DDAB) onto the glassy carbon electrode surface. Direct electron transfer of immobilized CPO was observed from cyclic voltammogram. The CPO modified electrode was further used as the working electrode for electrocatalytic chlorination of bergenin in O2-saturated bergenin solution containing potassium chloride. The chlorinated product was confirmed by HPLC-MS. The total turnover number(TTN) of the reaction is estimated to be 13600. |
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| Keywords: | Chloroperoxidase Modified electrode Bergenin Biocatalysis Electrochemical catalysis Chlorination |
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