水凝胶微流控芯片的快速加工及在细胞培养检测中的应用

采用具有紫外光聚合性能的聚乙二醇(PEG)基水凝胶材料, 通过紫外光聚合作用快速加工双层水凝胶微流控芯片, 并验证了其对肿瘤细胞代谢液进行检测的可行性. 与传统微流控芯片材料相比, 该水凝胶芯片材料具有更好的生物相容性及可操控性, 可直接加工成形, 在生物学领域特别是细胞培养过程控制方面具有良好的应用前景. 实验结果表明, 该水凝胶微流控芯片可在微尺度空间有效模拟细胞生长环境, 并实现对细胞连续捕获后的原位培养. 将该芯片与卟啉可视阵列传感器系统结合, 经代谢特征分析可有效区分不同种类肿瘤细胞, 实现芯片细胞培养平台上的细胞代谢指纹快速可视化传感检测.

Rapid Fabrication of Hydrogel Microfluidic Chip and Its Application for Cell Metabolites Detection

Taking advantage of the ultraviolet polymerization characteristic of poly(ethylene glycol) diacrylate(PEGDA) hydrogel material, a double-layer hydrogel microfluidic chip was fabricated rapidly by photopolymerization method. Moreover, feasibility of the chip culture for tumor cell metabolism detection was validated. The hydrogel material with better biocompatibility and handleability compared to traditional microfluidic chip material could be directly processed to obtain desirable micro-structure. The experimental results showed that the proposed method could achieve the fabrication of double-layer cell culture and detection chip with short time treatment. Cells could be well captured and cultured in the hydrogel microfluidic chip with excellent activity. Chip integrated with porphyrin colorimetric sensor array system could effectively distinguish different types of tumor cell by metabolism characteristics detection. The hydrogel microfluidic chip has the potential of practicable application once large-scale preparation is accomplished.