Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe |
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| Authors: | Mikhail Panfilov Darya Chernova Irina Khalfina Alexander Moskalensky Aleksey Vorob&#x;ev |
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| Institution: | 1.Novosibirsk Institute of Organic Chemistry, 9 Lavrentiev Avenue, 630090 Novosibirsk, Russia; (M.P.); (I.K.);2.Department of Physics, Novosibirsk State University, 630090 Novosibirsk, Russia; (D.C.); (A.M.) |
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| Abstract: | Nitric oxide (NO) is an important signaling molecule involved in a wide range of physiological and pathological processes. Fluorescent imaging is a useful tool for monitoring NO concentration, which could be essential in various biological and biochemical studies. Here, we report the design of a novel small-molecule fluorescent probe based on 9(10H)acridone moiety for nitric oxide sensing. 7,8-Diamino-4-carboxy-10-methyl-9(10H)acridone reacts with NO in aqueous media in the presence of O2, yielding a corresponding triazole derivative with fivefold increased fluorescence intensity. The probe was shown to be capable of nitric oxide sensing in living Jurkat cells. |
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| Keywords: | nitric oxide probes 9(10H)acridone fluorescent probes |
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