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Exploration of Hypoglycemic Activity of Saccharomyces pastorianus Extract and Evaluation of the Molecular Mechanisms
Authors:Chien-Hui Wu  Chung-Hsiung Huang  Ming-Chuan Chung  Shun-Hsien Chang  Guo-Jane Tsai
Affiliation:1.Department of Seafood Science, National Kaohsiung University of Science and Technology, Nanzi 81157, Taiwan;2.Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan; (C.-H.H.); (M.-C.C.);3.Institute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung 20224, Taiwan;4.Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan
Abstract:Although the hypoglycemic potential of brewer’s yeast extract has been reported, there is limited information pertaining to the hypoglycemic ingredients of Saccharomyces pastorianus extract and their mechanisms of action available. This study aimed to investigate the in vivo and in vitro hypoglycemic effect of S. pastorianus extract and to elucidate its molecular mechanisms. S. pastorianus extract was mainly composed of proteins followed by carbohydrates. In diabetic rats, oral administration of S. pastorianus extract significantly reduced the levels of plasma glucose and enhanced the activity of hepatic glucose-6-phosphatase dehydrogenase. Treatment with S. pastorianus extract increased the localization of type 4 glucose transporter (GLUT4), PTP, and insulin receptor at 3T3-L1 cell membranes and raised the levels of P38 MAPK, PI3K, and AKT in the cytosol. In agreement with these results, pretreatment of 3T3-L1 cells with inhibitors of PTP, PI3K, Akt/PKB, and p38 MAPK inhibited glucose uptake induced by application of S. pastorianus extract. Most importantly, a 54 kDa protein with hypoglycemic activity was identified and suggested as the major ingredient contributing to the hypoglycemic activity of S. pastorianus extract. In summary, these results clearly confirm the hypoglycemic activity of S. pastorianus extract and provide critical insights into the underlying molecular mechanisms.
Keywords:diabetes   glucose-6-phosphate dehydrogenase   type 4 glucose transporter   hypoglycemic protein   Saccharomyces pastorianus
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