| 测定葡萄糖的简易分光光度法 |
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| 引用本文： | 王长发,熊松,向阳,吴志强,蒋勉.测定葡萄糖的简易分光光度法[J].分析试验室,2001,20(1):17-18. |
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| 作者姓名： | 王长发 熊松 向阳 吴志强 蒋勉 |
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| 作者单位： | 武汉大学化学系, |
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| 摘 要： | 研究发现葡萄糖与磷钼杂多酸反应 ,能生成有色产物 ,它的最大吸收波长是 690 nm,吸光度与葡萄糖的质量浓度呈线性关系 ,检出限为 2× 1 0 -5mol/L。本法已用于测定葡萄糖注射液中的葡萄糖含量。
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| 关 键 词： | 葡萄糖 磷钼杂多酸 分光光度法 测定 葡萄糖注射液 |
| 文章编号： | 1000-0720(2001)01-0017-02 |
Simple Method for Determination of Glucose by Spectrophotometry |
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| WANG Chang-fa,XIONG Song,XIANG Yang,Wu Zhi-qiang,JIANG Mian.Simple Method for Determination of Glucose by Spectrophotometry[J].Chinese Journal of Analysis Laboratory,2001,20(1):17-18. |
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| Authors: | WANG Chang-fa XIONG Song XIANG Yang Wu Zhi-qiang JIANG Mian |
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| Abstract: | A spectrophotometric method for determination of glucose was developed based on measurement of color product formed by reaction of glucose with 12-molybdophosphate. The absorbance is proportional to concentration of glucose over the range of 2×10-5～2×10-3 mol/L with detection limit of 2×10-5 mol/L at wavelength of 690 nm.The method was applied to determination of glucose in injectors with satisfactory results. |
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| Keywords: | Glucose 12-Molybdophosphate Spctrophotometry |
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