LNA (locked nucleic acid) and analogs as triplex-forming oligonucleotides |
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Authors: | Højland Torben Kumar Surender Babu B Ravindra Umemoto Tadashi Albaek Nanna Sharma Pawan K Nielsen Poul Wengel Jesper |
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Affiliation: | Nucleic Acid Center, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark. |
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Abstract: | The triplex-forming abilities of some conformationally restricted nucleotide analogs are disclosed and compared herein. 2'-Amino-LNA monomers proved to be less stabilising to triplexes than LNA monomers when incorporated into a triplex-forming third strand. N2'-functionalisation of 2'-amino-LNA monomers with a glycyl unit induced the formation of exceptionally stable triplexes. Nucleotide analogs containing a C2',C3'-oxymethylene linker (E-type furanose conformation) or a C2',C4'-propylene linker (N-type furanose conformation) had no significant effect on triplex stability proving that conformational restriction per se is insufficient to stabilise triplexes. |
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