| 流动注射化学发光法测定中药材中没食子酸 |
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| 引用本文： | 方卢秋,李海芳,郑国金. 流动注射化学发光法测定中药材中没食子酸[J]. 分析化学, 2010, 38(8). DOI: 10.3724/SP.J.1096.2010.01110 |
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| 作者姓名： | 方卢秋 李海芳 郑国金 |
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| 作者单位： | 长江师范学院化学化工学院,涪陵,408100;清华大学化学系,北京,100084;清华大学化学系,北京,100084;清华大学化学系,北京,100084;楚雄医药高等专科学校公共部,楚雄,675000 |
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| 基金项目： | 重庆市教育委员会科学技术研究项目(No.KJ071304)基金 |
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| 摘 要： | 建立快速简便的流动注射化学发光分析法,用于中药材中没食子酸含量的测定.利用酸性介质中Fe3+-H2O2体系生成羟基自由基氧化没食子酸产生微弱的化学发光,用罗丹明6G来增敏化学发光.研究了影响化学发光的各种因素,探讨了可能的机理.结果表明,0.18 mol/L HCl,0.04 mol/L FeCl3,1.0 mol/L H2O2与1.0×10-4 mol/L罗丹明6G溶液组成最优的化学发光体系,没食子酸浓度在1.0×10-5～1.0×10-2 g/L和0.01～1.0 g/L范围内与化学发光强度呈很好的线性关系,r分别为0.9984和0.9947,检出限为3.0×10-6 g/L.对1.0×10-4 g/L没食子酸平行测定11次的相对标准偏差为3.8%.利用本方法成功地测定了中药材诃子和没食子中的没食子酸含量.
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| 关 键 词： | 中药材 没食子酸 流动注射 化学发光 罗丹明6G |
Determination of Gallic Acid in Chinese Herbal Medicine with Flow Injection Chemiluminescence Method |
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| FANG Lu-Qiu,LI Hai-Fang,ZHENG Guo-Jin. Determination of Gallic Acid in Chinese Herbal Medicine with Flow Injection Chemiluminescence Method[J]. Chinese Journal of Analytical Chemistry, 2010, 38(8). DOI: 10.3724/SP.J.1096.2010.01110 |
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| Authors: | FANG Lu-Qiu LI Hai-Fang ZHENG Guo-Jin |
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| Affiliation: | FANG Lu-Qiu1,2,LI Hai-Fang 2,ZHENG Guo-Jin2,3 1(Department of Chemical Engineering and Chemistry,Yangtze Normal University,Fuling 408100) 2(Department of Chemistry,Tsinghua University,Beijing 100084) 3(Common Course Department,Chuxiong Medical College,Chuxiong 675000) |
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| Abstract: | The rapid and easy flow injection chemiluminescence method was established to measure the content of gallic acid in the chinese herbal medicines. It was based on the enhanceed chemiluminescent emitted during the oxidation of gallic acid by producing of hydroxy free radical with chemical reaction between Fe3+ and H2O2 solution in HCl medium condition in the presence of rhodmine 6G as energy transfer in chemiluminescence system,in which various factors influenced the chemiluminescence system were investigated... |
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| Keywords: | Chinese herbal medicine Gallic acid Flow injection Chemiluminescence Rhodamine 6G |
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