Improved rapid assay of plasma uric acid by short-end injection capillary zone electrophoresis |
| |
Authors: | Salvatore Sotgia Ciriaco Carru Bastianina Scanu Elisabetta Pisanu Manuela Sanna Gerard Aime Pinna Leonardo Gaspa Luca Deiana Angelo Zinellu |
| |
Institution: | (1) Department of Biomedical Sciences and Centre of Excellence for Biotechnology Development and Biodiversity Research, University of Sassari, Viale San Pietro 43, 07100 Sassari, Italy;(2) Department of Medicinal and Toxicological Chemistry, University of Sassari, 07100 Sassari, Italy;(3) National Laboratory of the National Institute of Biostructures and Biosystems, 07033 Osilo, Italy |
| |
Abstract: | A rapid and simple short-end injection capillary zone electrophoresis method was developed for the quantification of plasma
uric acid. The separation was performed in an uncoated fused-silica capillary (50 μm ID, 60 cm total length, 10.2 cm effective
length) by using as a background electrolyte a 75 mmol/L glycylglycine solution titrated with NaOH 5 mol/L to pH 9.0, a voltage
of 28 kV, a cartridge temperature of 15 °C, and direct UV detection at 292 nm. Under optimized conditions, uric acid was determinate
in little more than 1 min (1.076 minutes). In order to verify the accuracy of the analysis, urate levels were measured in
543 apparently healthy volunteers by the new assay and our previous method, and the obtained data were compared by Passing–Bablock
regression, Bland–Altman test, and a new regression-based approach, which showed a good agreement between two methods. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|