基于高效分离不皂化物的傅里叶红外光谱结合化学计量学鉴定芝麻油

芝麻油是日常生活中常用食用油之一,掺假芝麻油会导致严重的健康问题.研究芝麻油鉴定方法是非常重要的.皂化植物油提取不皂化物是食用油鉴定的经济方法之一,现有植物油皂化方法需要较长时间,较高的温度,且不皂化物提取过程非常繁琐.采用超声技术替代常规回流加热法,提高了皂化效率,皂化时间缩短至10分钟,在此基础上采用专用固相萃取(...

Efficient Saponification and Separation of Unsaponifiables for Authentication of Sesame Oil Using FT-IR Spectroscopy and Chemometrics

Sesame oil is one of the essential cooking oil,and it has been consumed in daily life.The intake of adulterated sesame oil leads to severe health problems.Thus the method to identify adulteration is significant research.Saponification is one of the simple and inexpensive processes have been used to identify the adulteration in edible oil.The saponification takes a long time,higher temperature and the isolation of unsaponifiable from saponifiable is tedious.In the present research,the enriched saponification process has been developed using ultrasonication technique instead of a common conventional method.The process has been significantly reduced to ten minutes.The special solid phase extraction(SPE)cartridge has been designed and prepared to separate the unsaponifiable.The combined FT-IR with chemometrics based on the isolated unsaponifiable was first used to authenticate sesame oil.The partial least squares discriminant analysis(PLS-DA)and orthogonal partial least squares discriminant analysis(OPLS-DA)was employed to establish the models to identify the adulteration detection and authentication of sesame oil.The results indicated that the OPLS-DA is better than PLS-DA,which was chosen for the authentication of sesame oil.The prediction of samples was accurate by the constructed model.The results suggested that the combined FT-IR spectroscopy with chemometrics based on isolated unsaponifiable could be used for authentication of sesame oil.