| IgG免疫纳米探针凝聚反应压电传感检测 |
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| 引用本文： | 莫志宏,黄红稷,钱俊臻,朱丽华. IgG免疫纳米探针凝聚反应压电传感检测[J]. 高等学校化学学报, 2007, 28(4): 649-651 |
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| 作者姓名： | 莫志宏 黄红稷 钱俊臻 朱丽华 |
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| 作者单位： | 重庆大学化学化工学院,微系统研究中心,重庆,400044;重庆大学化学化工学院,微系统研究中心,重庆,400044;重庆大学化学化工学院,微系统研究中心,重庆,400044;重庆大学化学化工学院,微系统研究中心,重庆,400044 |
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| 摘 要： | IgG是人血清中主要抗感染的抗体, 约占成人血清免疫球蛋白总量的75%, 被视为判断健康或疾病的一个指标. 本文通过对IgG的检测, 探讨了纳米探针免疫凝聚压电传感方法的可行性.
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| 关 键 词： | 压电免疫传感 纳米探针 凝聚反应 IgG检测 |
| 文章编号： | 0251-0790（2007）04-0649-03 |
| 收稿时间： | 2006-05-12 |
| 修稿时间： | 2006-05-12 |
Piezoelectric Immunosensor Detection of IgG Based on Nanoprobe Immunoagglutination |
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| MO Zhi-Hong,HUANG Hong-Ji,QIAN Jun-Zhen,ZHU Li-Hua. Piezoelectric Immunosensor Detection of IgG Based on Nanoprobe Immunoagglutination[J]. Chemical Research In Chinese Universities, 2007, 28(4): 649-651 |
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| Authors: | MO Zhi-Hong HUANG Hong-Ji QIAN Jun-Zhen ZHU Li-Hua |
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| Affiliation: | College of Chemistry &; Chemical Engineering, Micro-System Research Center, Chongqing University, Chongqing 400044, China |
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| Abstract: | A piezoelectric immunoassay based on nanoprobe immunoagglutination was proposed for rapid and sensitive detection of IgG. The nanoprobes were prepared by labeling gold nanoparticles with anti-IgG antibody. And a piezoelectric sensor was used to monitor the changes in solution density and viscosity caused by the immunoagglutination of IgG and anti-IgG nanoprobes. The effects of pH and ionic strength were investigated. It is found that the developed immunoagglutination assay is sensitive to IgG with the detection limit of 1.12, 0. 72, 0. 70 nmol/L to the detection time of 10, 20, 30 min, respectively, and it is unnecessary for immobilization of immunoactive entities. |
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| Keywords: | Piezoelectric immunosensor Immuno-nanoprobe Immunoagglutination IgG detection |
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