| 配体交换色谱法测定草甘膦 |
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| 引用本文： | 石宏宇,张海柱,韩艳艳,龙远德,黄天宝. 配体交换色谱法测定草甘膦[J]. 分析化学, 2009, 37(5) |
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| 作者姓名： | 石宏宇 张海柱 韩艳艳 龙远德 黄天宝 |
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| 作者单位： | 1. 中国科学院成都有机化学研究所,成都,610041;中国科学院研究生院,北京,100049
2. 中国科学院成都有机化学研究所,成都,610041 |
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| 摘 要： | 建立了配体交换色谱测定草甘膦的方法.在自制的配体交换色谱柱上,以醋酸铜-醋酸水溶液为流动相,紫外(254 nm)检测,实现了草甘膦与双甘膦中间体的基线分离.考察了流动相中醋酸铜和醋酸的浓度,以及流动相流速对目标物分离的影响,确定了以0.2 mmol/L醋酸铜-醋酸水溶液为流动相,流速0.8 mL/min的优化色谱条件,并进行了系统适应性实验和方法学评价.结果表明:草甘膦与双甘膦之间的分离度Rs＞3,拖尾因子在0.8～1.0之间;在0.05～1.00 g/L范围内,草甘膦和双甘膦的线性相关系数分别为0 9998和0 9999; 回收率分别为102.2%和99.0%; 峰面积的RSD分别为0.14%和0.59%; 检出限分别为0 05和0.50 mg/L(S/N=3).
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| 关 键 词： | 配体交换色谱 草甘膦 双甘膦 |
Ligand-Exchange Chromatographic Method for Determining Glyphosate |
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| SHI Hong-Yu,ZHANG Hai-Zhu,HAN Yan-Yan,LONG Yuan-De,HUANG Tian-Bao. Ligand-Exchange Chromatographic Method for Determining Glyphosate[J]. Chinese Journal of Analytical Chemistry, 2009, 37(5) |
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| Authors: | SHI Hong-Yu ZHANG Hai-Zhu HAN Yan-Yan LONG Yuan-De HUANG Tian-Bao |
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| Affiliation: | Chengdu Institute of Organic Chemistry;Chinese Academy of Sciences;Chengdu 610041;Graduate School of the Chinese Academy of Sciences;Bejing 100049 |
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| Abstract: | A novel method for the determination of glyphosate by ligand-exchange chromatography was developed.Baseline separation between glyphosate and(N-phosphonomethyl) iminodiacetic acid(PMIDA) as an intermediate has been carried out on a homemade column using 0.2 mmol/L cupric acetate-0.2 mmol/L acetic acid as a mobile phase,flow rate 0.8 mL/min and detection at wavelength 254 nm.The chromatographic conditions,such as concentration of cupric acetate and acetic acid in the mobile phase as well as the flow rate,hav... |
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