| B SUBUNIT OF CHOLERA TOXIN PRODUCED IN Escherichia coli |
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| 引用本文： | 马清钧,刘传暄,熊凌霜,于秀琴. B SUBUNIT OF CHOLERA TOXIN PRODUCED IN Escherichia coli[J]. 中国科学B辑(英文版), 1991, 0(3) |
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| 作者姓名： | 马清钧 刘传暄 熊凌霜 于秀琴 |
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| 作者单位： | Bioemgineering Institute，Academy of Military Medical Sciences，Bioemgineering Institute，Academy of Military Medical Sciences，Bioemgineering Institute，Academy of Military Medical Sciences，Bioemgineering Institute，Academy of Military Medical Sciences Beijing 100850，PRC，Beijing 100850，PRC，Beijing 100850，PRC，Beijing 100850，PRC |
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| 摘 要： | An engineered E. coli strain containing high expression level of CT-B subunits has beenobtained by the application of recombinant DNA techniques. The B subunit can be secretedinto the medium and reaches 20- 40 μg/ml when this strain is incubated in a 50 1 fermenta-tion tank. The CT-B subunit purified with affinity chromatography in E. coli has the samecharacters as the natural CT- B subunit in molecular weight, N terminal amino acid analysisand antigenicity. The CT-B subunit has good immunogenicity and can be used as a preparation for protect-ing against diarrhea caused by V. cholera and enterotoxigenic E. coli. It can also be usedas a vector for hepatins.
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B SUBUNIT OF CHOLERA TOXIN PRODUCED IN Escherichia coli |
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| MA QING-JUN,LIU CHUAN-XUAN,XIONG LING-SHUANG,YU XIU-QIN Bioemgineering Institute,Academy of Military Medical Sciences,Beijing ,PRC. B SUBUNIT OF CHOLERA TOXIN PRODUCED IN Escherichia coli[J]. Science in China(Chemistry), 1991, 0(3) |
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| Authors: | MA QING-JUN LIU CHUAN-XUAN XIONG LING-SHUANG YU XIU-QIN Bioemgineering Institute Academy of Military Medical Sciences Beijing PRC |
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| Affiliation: | MA QING-JUN,LIU CHUAN-XUAN,XIONG LING-SHUANG,YU XIU-QIN Bioemgineering Institute,Academy of Military Medical Sciences,Beijing 100850,PRC |
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| Abstract: | |
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| Keywords: | E.coli cholera toxin |
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