HPLC-Methode zur simultanen Bestimmung von Harnstoff, Kreatinin und Harns?ure in Serum und Urin

Summary A simple, rapid, and reproducible method for the determination of urea, creatinine, and uric acid in human serum and urine by ion-pair reversed-phase high-performance liquid chromatography with UV detection (196 nm) has been developed. The method involves the pretreatment of serum samples with trichloroacetic acid and centrifugation followed by the isocratic separation of compounds on a -Bondapak C18 column using a mobile phase consisting of 1.25 mmol/l tetrabutylammonium phosphate. For urine samples no special pretreatment is necessary. In addition, this method allows, with some limitations, the determination of creatine in serum.