| 低盐即食虾皮氨含量的高效液相色谱分析方法 |
| |
| 引用本文： | 柳晓萍,郝更新,杨燊,孙乐常,石林凡,任中阳,邱绪建.低盐即食虾皮氨含量的高效液相色谱分析方法[J].集美大学学报(自然科学版),2022,27(2):133-137. |
| |
| 作者姓名： | 柳晓萍 郝更新 杨燊 孙乐常 石林凡 任中阳 邱绪建 |
| |
| 作者单位： | （集美大学海洋食品与生物工程学院，福建 厦门 361021） |
| |
| 摘 要： | 建立低盐即食虾皮中氨含量的高效液相色谱(high performance liquid chromatography,HPLC)分析方法.样品粉碎经三氯乙酸提取后,用丹磺酰氯衍生并用甲苯净化提取,然后采用乙腈和水梯度洗脱,254 nm紫外检测.结果表明,内标法的线性范围是0.0005～0.2 g/L,回收率为84％ ～...
|
| 关 键 词： | 虾皮 生物胺 氨 低盐 高效液相色谱 |
Determination of Ammonia in Low Salt Ready-to-Eat Dried Shrimp (Acetes chinensis) by HPLC Method |
| |
| LIU Xiaoping,HAO Gengxin,YANG Shen,SUN Lechang,SHI Linfan,REN Zhongyang,QIU Xujian.Determination of Ammonia in Low Salt Ready-to-Eat Dried Shrimp (Acetes chinensis) by HPLC Method[J].the Editorial Board of Jimei University(Natural Science),2022,27(2):133-137. |
| |
| Authors: | LIU Xiaoping HAO Gengxin YANG Shen SUN Lechang SHI Linfan REN Zhongyang QIU Xujian |
| |
| Institution: | (College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China) |
| |
| Abstract: | A high performance liquid chromatography(HPLC) method for determination of biogenic amines was developed and validated to determine ammonia content in low salt ready to eat dried shrimp samples.Samples were ground and mixed with trichloroacetic acid to extract ammonia.Then the extract was derivatized by dansyl chloride and extracted by toluene before analysis by HPLC.The results showed good linearity and repeatability in the range of 0.000 5-0.2 g/L.The recovery rate was 84%-105%.The detection limit in samples was approximately 0.7 mg/L.The HPLC method could also simultaneously analyze the possible biogenic amines generated in the samples. |
| |
| Keywords: | dried shrimp biogenic amine ammonia low salt high performance liquid chromatography(HPLC) |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《集美大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《集美大学学报(自然科学版)》下载免费的PDF全文 |
