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LC with Column-Switching and Spectrophotometric Detection for Determination of Risperidone and 9-Hydroxyrisperidone in Human Serum |
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| Authors: | Katrin M Kirschbaum Susann Finger Friederike Vogel Rainer Burger Manfred Gerlach Peter Riederer Christoph Hiemke |
| Institution: | (1) Department of Psychiatry, University of Mainz, 55131 Mainz, Germany;(2) Department of Psychiatry, University of Würzburg, 97080 Würzburg, Germany;(3) Department of Child and Adolescent Psychiatry, University of Würzburg, 97080 Würzburg, Germany |
| Abstract: | An automated high performance liquid chromatography with column-switching and ultraviolet detection was developed for the analysis of risperidone and 9-hydroxyrisperidone. The method needs minimum sample preparation and is useful for the detection down to a limit of 1 ng mL−1. Sample clean-up of serum was carried out on a CN 20 μm SPE-column using 8% (v/v) acetonitrile in water. Chromatographic separation was performed on ODS Hypersil C18 material with 38% (v/v) acetonitrile and 0.4% (v/v) TEMED in water. Application of the method to the analysis of serum samples confirmed its suitability for therapeutic drug monitoring of risperidone and 9-hydroxyrisperidone. |
| Keywords: | Column liquid chromatography Ultraviolet detection Column-switching Therapeutic drug monitoring Risperidone and 9-hydroxyrisperidone |
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